Tune without dyno

Is it possible to tune/ reflash without dyno? How will i know the perfect ignition timing and there the MBT is? Or i can found that info in the reflash software? Also, how will i know the perfect AFR without Dyno?

It is practically impossible to optimally tune ignition timing without a dyno. You need to measure the results with a dyno. However, with reflash turning, often the OEM has close to optimum timing already, so there is often no need to make many changes. With a stand alone ECU starting from scratch, best to have a plan for dyno sessions to get the ignition right.

HPA has some info on road tuning specifically which I think relates to your interest.

https://www.hpacademy.com/courses/road-tuning/